Larin has scored eight goals in 17 matches for Orlando in MLS this season and joins a Canadian side that netted five in its three group matches.
The 22-year-old has five goals for his country in 20 appearances.
Canada finished second in Group A behind Costa Rica and will face the runner-up from Group C at the University of Phoenix Stadium in Glendale, Arizona, on Thursday.
Larin missed most of June after being arrested on a DUI charge, sitting out three games for Orlando before being cleared to return to action by MLS' Substance Abuse and Behavioral Health Program.
CANADA 23-PLAYER KNOCKOUT ROUND SQUAD
Goalkeepers (3): Maxime Crepeau (Montreal Impact), Milan Borjan (MKS Korona Kielce / POL), Jayson Leutwiler (Shrewsbury Town FC / ENG)
Defenders (6): Manjrekar James (Vasas Budapest / HUN), Steven Vitoria (Lechia Gdansk / POL), Dejan Jakovic (New York Cosmos / USA), Samuel Adekugbe (Vancouver Whitecaps), Adam Straith (FC Edmonton), Marcel de Jong (Vancouver Whitecaps)
Midfielders (11): Fraser Aird (unattached), Samuel Piette (CD Izarra / ESP), Russell Teibert (Vancouver Whitecaps), Scott Arfield (Burnley FC / ENG), , David Junior Hoilett (Cardiff City FC / WAL), Alphonso Davies (Vancouver Whitecaps), Jonathan Osorio (Toronto FC), Mark-Anthony Kaye (Louisville City FC / USA), Anthony Jackson-Hamel (Montreal Impact), Patrice Bernier (Montreal Impact), Michael Petrasso (Queens Park Rangers / ENG)
Forwards (3): Lucas Cavallini (CA Penarol / URU), Cyle Larin (Orlando City / USA), Tosaint Ricketts (Toronto FC)