Zinedine Zidane's side are second in the table, below Barcelona due to their inferior head-to-head record in the meetings between the two teams this season, but with the same points as the Catalan club and still with a game in hand over their Clasico rivals.
Madrid are in action against Sevilla in what will be their last home match of the campaign, while Barca kick off at the same time away to Las Palmas.
REAL MADRID INJURIES
Gareth Bale is still sidelined with a calf problem, while Dani Carvajal is also out after suffering a hamstring injury in the first leg of his side's Champions League semi-final win over Atletico last week.
Pepe is training normally and could return to the squad.
REAL MADRID SUSPENSIONS
Cristiano Ronaldo, Carvajal (out injured anyway) and Isco are all just one yellow card away from a ban, but Zidane has no players suspended for the game against Sevilla.
REAL MADRID POTENTIAL STARTING LINE-UP
There are no more Champions League matches now until the final on June 3, but Real Madrid are in action against Celta Vigo in midweek. Nevertheless, Zidane is set to field a strong side against Sevilla as Los Blancos look to close in on the title.
The Frenchman has rested players in recent weeks and is likely to rotate his pack again for this Liga clash, although Cristiano Ronaldo should start in attack after sitting out the last two Primera Division matches.
There should also be a place in the team for James Rodriguez, who has netted five goals in his last four appearances in La Liga and has also scored in each of his seasons at the club against Sevilla.
SEVILLA TEAM NEWS
Sevilla are in fourth place in La Liga and need just one more point to guarantee a top-four finish as the Andalusians are six points clear of Villarreal with just two fixtures remaining.
Sampaoli's side have enjoyed a spectacular season, although results have dipped in recent weeks and the team is without an away win since beating Betis 2-1 in the Seville derby on February 25.
Vitolo and Sergio Escudero are back in training and should feature at the Bernabeu, but Vicente Iborra, Mariano Ferreira and Adil Rami are all doubtful. Pablo Sarabia misses out through suspension.
BEST OPTA MATCH FACTS
The last Sevilla win against in La Liga against Real Madrid at the Santiago Bernabeu was in December 2008 (4-3). Since then, the Andalusians have lost in each of their seven visits.
Real Madrid are the only team to have scored six or more goals in a Liga game against Sevilla in the last 10 years (three times).
This season, Madrid won against Sevilla in the UEFA Super Cup and also knocked them out of the Copa del Rey.
With their win in the reverse fixture (2-1), Sevilla ended the longest unbeaten run by a La Liga side in all competitions, recorded by Real Madrid (40 games).
Cristiano Ronaldo has scored 23 goals against Sevilla in all competitions, his favourite opponent in terms of goals scored.
UK TV COVERAGE & KICK-OFF TIME
Real Madrid versus Sevilla kicks off at 20:00 CEST at the Santiago Bernabeu and will be shown live from 18:30 UK time on Sky Sports 3.