Uefa has upheld its decision to deduct one point from Croatia's Group H Euro 2016 qualification tally for the display of a swastika during a game in June.
The European football governing body punished the side after the fans used a chemical agent to imprint a swastika on the pitch of Split's Poljud stadium before their 1-1 draw with Italy.
The Nazi symbol became visible during the match and Croatia were ordered to play their next two competitive home games behind closed doors and fined €100,000.
"Uefa's appeals body has upheld the decision to dock Croatia one point for racist behaviour at their qualifying Group H game against Italy," the Uefa statement read on Friday.
"The appeal by Croatia's football federation (HNS) was rejected."
The decision means Croatia are third in Group H on 14 points from eight games, four behind leaders Italy and two adrift of second-placed Norway.
Should they finish third following the completion of the remaining two games then they could still reach France via the play-offs.