A visibly emotional Themba Zwane walked off the pitch after just 20 minutes of being introduced from the bench in the Premier Soccer League match against Magesi FC, which Masandawana won 3-0 at Lucas Moripe on Sunday.
It was his first match since returning from a thigh injury, which he sustained against Orlando Pirates in the first leg of the MTN8 semi-finals in August.
Masandawana coach Miguel Cardoso has commented on the latest fitness setback his captain has suffered.