This weekend's meeting will be the third time the two teams are playing each other in the ongoing edition across all competitions.
The first encounter was in the PSL and Masandawana won 2-1.
The latter then defeated the Glamour Boys 4-0 in the Carling Knockout quarter-final fixture.
Ahead of the Saturday match, Downs will be without three key players.
Aubrey Modiba and Denis Onyango are suspended, and coach Miguel Cardoso confirmed first-choice keeper Ronwen Williams is also out.
Onyango's ban has been extended to three matches as he has picked up two red cards this season.