Juventus striker Gonzalo Higuain was taken off just 15 minutes into the derby clash with Torino after picking up an injury.
The Argentina international landed heavily five minutes into Sunday's match after hurdling goalkeeper Salvatore Sirigu when trying to reach Miralem Pjanic's throughball.
The 30-year-old tried to continue after receiving treatment but eventually had to be replaced by Federico Bernardeschi, with it appearing that Higuain had hurt his ankle.
It will come as a concern for Juve boss Massimiliano Allegri ahead of a crucial run of fixtures in his side's season.
Juve take on Atalanta in Serie A next Sunday before the second leg of the Coppa Italia semi-final against the same opponents three days later.
They then begin March with a trip to Lazio, four days before they take on Tottenham in the Champions League last 16, who hold the edge in the tie on away goals after a 2-2 draw in Turin.
The loss of the Argentine, who scored both of his side's goals against Spurs on Tuesday, was a big blow for the Serie A champions as they look to overtake Napoli at the top of the table.
However, Bernardeschi proved to be a sound replacement for the former Real Madrid and Napoli star as he took on a pass from Alex Sandro and drove into the box to set the Brazilian up with the return pass and give his side the lead after 33 minutes.