Struggling to overcome a dogged Sporting side, Diego Simeone's men were deadlocked at 1-1 when the Frenchman scored his first goal after 80 minutes.
But, if Atleti thought that was the highlight of the game, they were mistaken, as Gameiro embarked on an incredible scoring run to kill off the match.
4'43" - @kevingameiro9 has scored the fastest hat-trick in La Liga since Bebeto's hat-trick vs Albacete in 1995. Milestone.— OptaJose (@OptaJose) 18 de febrero de 2017
The ex-Sevilla man netted his second moments later with a cool finish, and rounded off his hat-trick in the 85th minute by converting from close range.
His effort is the fastest recorded triple in the history of the division, although the list is not definitive.
Bebeto, for example, netted three times in three minutes back in 1995 to take Albacete apart, a hat-trick that has not been officially timed.
Gameiro's three goals came in just four minutes and 43 seconds to surpass the mark of David Villa in the overall standings.
Gameiro's devastating run sealed a 4-1 win for Atletico, who remain in touch with the Liga front-runners four points shy of Real Madrid, albeit having played three more games than their city rivals.