Doubts over the 24-year-old's fitness first arose on Wednesday and those were realised following a scan, which showed minor damage to the forward's left hamstring.
Spurs are unwilling to risk the Premier League's top scorer for the Old Trafford date, aware that the injury could be made worse if he were to play too soon.
"We cannot take a risk for tomorrow, we'll see after for Wednesday. But tomorrow, it is impossible," manager Mauricio Pochettino confirmed during his press conference.
Spurs also offered an update on the fitness of Kenya midfielder Victor Wanyama. The former Celtic and Southampton man is "receiving treatment and continuing to go through rehabilitation, following up with a specialist next week".
Spurs find themselves level with Man Utd on points ahead of the weekend's clash, though they trail Jose Mourinho's side on goal difference. Both clubs lag league leaders Manchester City by five points, however.
HOW DO SPURS PERFORM WITHOUT KANE?
Kane has been an ever present for Pochettino in both the league and Europe this season, sitting out only a couple of EFL Cup fixtures, including the 3-2 defeat against West Ham in midweek.
Last season, an ankle injury saw him miss several weeks towards the beginning of the season. Although Spurs only suffered two defeats during this absence - those to Bayer Leverkusen in the Champions League and Liverpool in the EFL Cup, a game he probably would not have played in any case - they were only able to win two of their five league fixtures, although one of these did come in a 2-0 home victory over Manchester City.
Meanwhile, a similar issue hampered the forward from mid-March until the beginning of April. During this time, Spurs recorded three wins from three, defeating Southampton, Burnley and Swansea.