Donovan, the United States and MLS all-time leading scorer, told Yahoo Sports that he is officially closing the door on his playing career.
"Yeah, I'm done," Donovan said. "I'm done. No more playing for me."
"I have not [gone public]. But that is definitely the case."
Donovan, 34, retired in December 2014 after winning his record sixth MLS Cup with the LA Galaxy, but he returned to the pitch last September to help the injury-riddled Galaxy fight for a playoff spot. The midfielder ended the season with one goal in nine appearances.
Donovan tallied 160 goals and 151 assists in 384 appearances over his MLS career. He scored 57 times and dished out 57 assists in 157 matches for the U.S.