Police in full riot gear used tear gas to disperse fans who had congregated in the French port on Friday following similar clashes on Thursday night.
There were reports of bottles being thrown at police by fans who had been drinking with police using tear gas and detaining some fans in a bid to restore order.
Goal's Chris Wheatley was on the scene and said: "We’ve just seen someone knocked out. Absolutely shocking scenes here in Marseille. I can still feel the tear gas in my eyes. There’s not much else to say apart from absolutely disgraceful scenes by England fans.
"We were told to put our cameras down by some England fans. Have they destroyed anything yet? It looks like they are about to. Looking at all the shopkeepers packing way now. Absolutely disgraceful scenes now.
"Shocking scenes in Marseille. Riot police are here but obviously there's not much they can do about it as there are thousands of England fans on the streets about 70,000 predicted to be at Stade Velodrome for Saturday's game now about 12 hours away. "
After Thursday’s disturbances the Football Association released a statement asking for supporters to behave as Roy Hodgson’s men prepare for their first game of the tournament.
“We are really disappointed by the scenes of disorder in Marseille yesterday evening and condemn such behaviour,” read the statement.
“It is in the hands of the authorities to identify those involved and deal with them appropriately.
“The FA urges supporters who are travelling to Marseille to act in a respectful manner and enjoy the match against Russia.”