Brazil will meet their hosts at Eden Park Stadium on March 26 in one of their first two matches of the year.
The other fixture during the March 2019 FIFA International period will be announced in due course.
Their last meeting came at the 1997 FIFA Confederations Cup as goals from Romario and Ronaldo secured a 2-0 semi-final victory as Brazil went on to lift the title.
Following March’s action, the Brasil Global Tour will resume in June as Tite and his squad play their final friendlies before embarking on their Copa America campaign.
Czech Republic vs Brazil
Tuesday 26 March 2019
Eden Park Stadium, Prague
Kick off 20:45 Local Time (CET) TBC
Kick off 19:45 UK Time (GMT) TBC
Kick off 16:45 Rio Time (BRT) TBC