Theo Walcott and Jack Wilshere start for Arsenal in their Europa League encounter with Red Star Belgrade on Thursday.
Mathieu Debuchy, meanwhile, makes his first Gunners start in nearly a year while Mohamed Elneny starts alongside Holding and the Frenchman in a makeshift defence.
The Gunners made easy work of Cologne and BATE Borisov in their first two group-stage matches, and can move five points clear at the top of Group H with a win on Thursday.
However, they are without a number of first team stars including Mesut Ozil and Alexis Sanchez while Chuba Akpom is the most recognisable name among Arsene Wenger's subs.
Arsenal XI: Cech; Debuchy, Elneny, Holding; Nelson, Coquelin, Willock, Maitland-Niles; Walcott, Wilshere; Giroud.
Subs: Macey, DaSilva, Nketiah, Akpom, McGuane, Osei-Tutu, Sheaf.