Tierna Davidson suffered a knock during the first half of the USWNT's win over Germany on Sunday, forcing her out of the game with Emily Sonnett coming on in her place.
The USWNT central defender will now miss Wednesday's group stage match against Australia, U.S. Coach Emma Hayes confirmed Tuesday.
The injury leaves the U.S. without two players for the final group stage match. Davidson will join the sidelined Jaedyn Shaw, who has yet to play at the tournament due to her own leg injury. Shaw will miss her third straight Olympics match.