Luis Enrique faces a minor defensive crisis for the Liga finale, with Pique joining Javier Mascherano and Jeremy Mathieu on the casualty list.
The coach therefores turns once more to the ex-Fluminense man, 21, who joined earlier this season and made his Liga debut during last week's win over Las Palmas.
Samuel Umtiti partners Marlon in defence, while Sergi Roberto lines up outside at right-back.
The rest of the Catalan first XI has a familiar look, with Luis Enrique's midfield and attack at full strength.
Barcelona: Ter Stegen; Roberto, Marlon, Umtiti, Alba; Busquets, Rakitic, Iniesta; Messi, Suarez, Neymar. Subs: Cillessen, Denis, Arda, Paco Alcacer, Digne, Andre Gomes, Borja Lopez.