They have been drawn alongside Belarusian side BATE Borisov and Hungary's MOL Vidi as well as PAOK and will be aiming to get off on the right foot with three points.
With a potential route into next season's Champions League the reward for the tournament winners, there is plenty at stake for the Stamford Bridge club in this competition.
|Game||PAOK vs Chelsea|
|Date||Thursday, September 20|
|Time||5:55pm BST / 12:55pm ET|
TV Channel, Live Stream & How To Watch
In the United States (US) the game will be available to watch live on television via UniMas and it can be streamed live online using Univision NOW or B/R Live.
|US TV channel||Online stream|
|UniMas||Univision NOW / B/R Live|
The game can be watched live on television in the United Kingdom (UK) via BT Sport 2 and it can be streamed live online using the BT Sport app.
|UK TV channel||Online stream|
|BT Sport 2||BT Sport app|
Squads & Team News
|Goalkeepers||Rey, Paschalakis, Siampanis, Balomenos|
|Defenders||Matos, Varela, Tosca, Malezas, Crespo, Vieirinha, Lyratzis, Khacheridi, Tsaousis, Michailidis, Diamantis, Kitsiou|
|Midfielders||El Kaddouri, Mauricio, Pelkas, Limnios, Wernbloom, Biseswar, Shakhov, Chatistravos, Karamperis, Tsiggaras, Maniotis, Gaitanidis, Warda, Canas|
|Forwards||Prijovic, Karelis, Gkargkalatzidis, Tzovaras, Akpom, Jaba|
Defender Leo Matos is suspended for the game, but Rzvan Lucescu has a full panel available, including former Arsenal striker Chuba Akpom.
PAOK XI: Paschalakis; Khacheridi, Varela, Tosca, Vieirinha; Wernbloom, Mauricio, El Kaddouri, Shakhov, Jaba; Pelkas.
|Goalkeepers||Kepa, Caballero, Bulka|
|Defenders||Rudiger, Alonso, Zappacosta, Cahill, Christensen, Azpilicueta|
|Midfielders||Fabregas, Jorginho, Kante, Barkley, Pedro, Loftus-Cheek, Ampadu, Hudson-Odoi, Moses, Willian|
Sarri brought a 21-man travelling party to Greece for the game and Eden Hazard is among the notable absentees, along with Mateo Kovacic, David Luiz and Emerson.
Cesc Fabregas has been included and could potentially make a return to action.
Chelsea XI: Kepa; Zappacosta, Rudiger, Christensen, Alonso; Jorginho, Kante, Barkley; Willian, Pedro, Morata.
Betting & Match Odds
Chelsea are 13/20 favourites to win the game according to bet365, who deem PAOK to be a 5/1 bet to beat the Blues. A draw is available at 29/10.
Alvaro Morata is favourite to open the scoring at 7/2, followed by Olivier Giroud at 9/2.
The reign of Sarri has begun in tremendous fashion, with Chelsea maintaining a 100 per cent record in their opening five matches of the season and the Italian coach is unsurprisingly well prepared for the game against PAOK.
"I have seen the four matches played by PAOK in the Champions League play-offs. My staff have seen the matches of the Greek championship. I think I know everything," Sarri told reporters ahead of the game.
"We want to play with our characteristics, to control the match with our ball possession, but I know [the game] will be difficult. They are a good team. They have won against Basel, Spartak Moscow, they drew against Benfica in Portugal. We have to do a very good match if we want to gain points."
The Blues will have to make do without their talisman Hazard, who has been allowed some extra time to recover following his hat-trick display against Cardiff City in the league, but Sarri has sufficient depth to cover for the Belgium international.
Willian and Pedro will be expected to fill the creative void left, but Sarri could also potentially use the game - and the group stage of the tournament in particular - as an arena in which to blood players such as Callum Hudson-Odoi and Ethan Ampadu, who have been making an impression.