The Argentine attacker had been a regular in the first-team under Mauricio Pochettino but a hip injury has kept him out since his last appearance on October 25, 2016, against Liverpool in the EFL Cup.
While Spurs' first-team will take on fierce rivals Arsenal in their crucial game early on Saturday afternoon, 25-year-old Lamela starts for the younger side in the Premier League 2 clash with another London side.
A return to action for @ErikLamela who starts for our U23s in the #PL2 today.
We face Chelsea at 12pm at Hotspur Way. Here’s our full line-up… #COYS pic.twitter.com/u7gnTIjqXN — Tottenham Hotspur (@SpursOfficial) November 18, 2017
Although Pochettino's team are looking good in third-place in the English top flight, Spurs U-23s are eighth in their 12-team division - one point ahead of Chelsea U23s.
Lamela has made 121 appearances for Tottenham since joining from Roma in 2013 and is contracted to the club until 2020.