Mauricio Pochettino's men came from two goals down in the first leg to secure a 2-2 draw in Turin in February and, as such, they will feel confident in their ability to progress.
The concession of two goals, particularly at home, was a rare experience for Juve, but they will arrive in London without trepidation, especially considering how inconsistent Spurs' form has been.
The tie is finely balanced and both sides will be equally determined to ensure their place in the last eight.
|Game||Tottenham Hotspur vs Juventus|
|Date||Wednesday March 7|
|Time||19:45 GMT / 14:45 ET|
|Stream (US only)||fubo TV (7-day free trial)|
TV Channel & Live Stream
In the United States (US), the game can be watched live and on-demand with fuboTV (7-day free trial).
New users can sign up for a free seven-day trial of the live sports streaming service, which can be accessed via iOS, Android, Chromecast, Amazon Fire TV, Roku and Apple TV as well as on a web browser.
|US TV channel||Online stream|
|Fox Sports 1||fubo TV (7-day free trial)|
In the United Kingdom (UK), the game can be watched live on TV on BT Sport 2 and it can be streamed live online using the BT Sport app.
|UK TV channel||Online stream|
|BT Sport 2||BT Sport app|
Squads & Team News
|Goalkeepers||Lloris, Gazzaniga, Vorm, Whiteman|
|Defenders||Trippier, Rose, Alderweireld, Vertonghen, Sanchez, Aurier, Davies, Walker-Peters|
|Midfielders||Lamela, Wanyama, Dier, Sissoko, Dembele, Alli, Eriksen, Lucas, Winks, Georgiou, Oakley-Boothe|
|Forwards||Son, Kane, Llorente, Sterling|
Tottenham don't have too many personnel concerns ahead of the game but defender Toby Alderweireld is out as he continues to recover from a thigh injury.
Serge Aurier is suspended for the match, so there are likely to be some alterations to the backline.
Potential Spurs starting XI: Lloris; Trippier, Vertonghen, Sanchez, Davies; Dier, Dembele, Alli, Eriksen, Son; Kane.
|Goalkeepers||Buffon, Szczesny, Pinsoglio|
|Defenders||De Sciglio, Chiellini, Benatia, Sandro, Barzagli, Rugani, Lichtsteiner|
|Midfielders||Pjanic, Khedira, Cuadrado, Marchisio, Costa, Matuidi, Asamoah, Sturaro, Bentancur, Bernardeschi, Muratore|
|Forwards||Higuain, Dybala, Mandzukic|
Juve will be without the services of Mattia De Sciglio, while Juan Cuadrado and Federico Bernardeschi are also expected to miss out.
Striker Gonzalo Higuain is a slight doubt for the match after sustaining a knock but the former Real Madrid man has taken part in training.
Potential Juventus starting XI: Buffon; Barzagli, Chiellini, Benatia; Lichtsteiner, Asamoah, Pjanic, Matuidi, Khedira; Higuain, Dybala.
Betting & Match Odds
Tottenham are at home in the second leg and are considered favourites by dabblebet, who price them at 23/20 to win. Juve, meanwhile, are priced at 12/5 to win in London and a draw is available at 23/10.
Spurs boast two away goals so they are favourites to qualify from the tie at 2/5, with Juve coming in at 15/8.
With Spurs boasting the away goal advantage from the first leg, Juventus' hopes of progressing from the last 16 rely on their ability to score more goals than their hosts on Wednesday.
And Bianconeri coach Massimiliano Allegri, whose team have not been beaten in any competition since last November, believes fully in his players' ability to do so, hinting at a vulnerability in the north London side's defence.
"Tottenham are different compared to Lazio, they don't defend as well," Allegri told reporters after his side's narrow victory over Lazio. "This win certainly helps our confidence as we will have to be good and lucky on Wednesday."
However, while it may be true that Pochettino's side have leaked goals - and against lesser opposition too - in recent weeks, it is also the case that they boast one of the most formidable strike-forces in Europe.
The likes of Giorgio Chiellini and co. are masters at shutting teams out, but they were unable to do so in Turin two weeks ago so will have to be on top of their game on Wednesday if they wish to overturn their hosts and make their way into the quarter-finals.