Pablo Machin's side won 1-0 at the Stadio Olimpico last week thanks to Wissam Ben Yedder. Lazio know they must score twice at a stadium where Sevilla have won 12 of their last 15 matches - including victories over Barcelona and Real Madrid.
bet365 offer Sevilla at 4/5 (1.80) to book their place in the last 16 of the competition they have won on five occasions with a win. Lazio are priced at 17/4 (5.25) to win in 90 minutes and 11/2 (5.50) to qualify. The draw is available at 11/4 (3.75).
Sevilla have winger Nolito out with a long-term injury, while Maxime Gonalons, Joris Gnagnon and Aleix Vidal are doubts.
Lazio are boosted by the return of both Sergej Milikovic-Savic and Luis Alberto from injury, while top scorer Ciro Immobile will also return for the Romans.
In last week's first leg we backed a low-scoring game and there could be a temptation to invest in a repeat; Sevilla have found goals a little harder to come by of late, while Lazio are far from prolific.
Usually the Andalusians are roared on at home on a European night by a passionate home crowd, but the game has been moved to the curious kick-off slot off 6pm local time because of local rivals Real Betis in European action on Thursday.
As such, the raucous atmosphere that is so often present at the Sanchez Pizjuan could be a little subdued.
Tips and predictions
Opposing an early goal is highly tempting, with the price of 5/6 (1.83) for no goal to be scored before the 28th minute looking generous.
Also worthy of consideration could be a goalless first half at 2/1 (3.00) in what could be a tight opening 45 minutes between two evenly-matched teams.
Odds correct at the time of writing. Please gamble responsibly.