The Falcons and True Parrots Team roster features Uniao de Madeira midfielder Jardel Nazare and SC Covilha striker Ludgerio Silva - two of nine players based in Portugal.
Sao Tome, who beat Mauritius to qualify for the group stage of the qualifiers, will first play as guests to Sudan on November 14 and host Ghana four days later in their first Group C games.
Only the top two nations in the group at the end of the qualifiers will secure a place at the final tournament in Cameroon in 2021.
Goalkeepers: Adalmir Almeida Primo (Praia Cruz, Sao Tome), Gilmar Eusebio Tavinho (Trindade FC, Sao Tome)
Defenders: Leonildo Soares Ju (Oriental Dragon, Portugal), Denilson Quaresma (UDRA, Sao Tome), Ivonaldo Mendes (UDRA, Sao Tome), Charles Monteiro (SG Sacavenense, Portugal), Edley Motoia Vava Pequeno (Praia Cruz, Sao Tome), Lucio Olivieira (FC Silves, Portugal), Diego Jordao (Needham Market FC, England)
Midfielders: Joazhifel Sousa Ponte (UDRA, Sao Tome), Marco Paulo Barbeiro (FC Massama, Portugal), Jardel Nazare (Uniao de Madeira, Portugal), Eduardo Varela (FC Porto Real, Sao Tome), Harramiz Soares (Leixoes SC, Portugal), Mauro Santos (Cova de Piedade, Portugal), Jokceleny Carvalho (UDRA, Sao Tome)
Attackers: Ludgerio Silva (SC Covilha, Portugal), Luis Anjos Leal (Newell's Old Boys, Argentina), Jeecimilson Tatty (FC Pampilhosa, Portugal)