After a goalless first half, the forward opened scoring at the AOK Stadion in the 53rd minute after being set up by Daniel Didavi.
Osimhen’s effort was neutralized when Jari Oosterwijk scored the equalizer for Gert Verbeek’s men seven minutes later.
Robin Knoche then secured the win for the Wolves in the 68th minute.
Osimhen who has played in two Bundesliga games this campaign was later replaced by youngster John Yeboah with fourteen minutes remaining in the encounter.
Wolfsburg sit on the 14th spot in the Bundesliga log and will host Freiburg at the Volkswagen Arena for their next league fixture on November 18.