Tuesday's meeting will be the three-time African champions' second of five friendly games before they open their 2018 World Cup campaign in June, though Serbia have no additional fixtures on their calendar between now and the opening of the tournament.
The game represents an opportunity for players to secure their place in their nation's respective World Cup squads in the coming months, if nothing else.
|Game||Nigeria vs Serbia|
|Date||Tuesday, March 27|
|Time||20:00 BST / 15:00 ET|
|Stream (US only)||fubo TV (7-day free trial)|
TV Channel & Live Stream
In the United States (US), the game can be watched live and on-demand with fuboTV (7-day free trial).
New users can sign up for a free seven-day trial of the live sports streaming service, which can be accessed via iOS, Android, Chromecast, Amazon Fire TV, Roku and Apple TV as well as on a web browser.
|US TV channel||Online stream|
|beIN Sports||fubo TV (7-day free trial)|
In the United Kingdom (UK), the game will not be available for broadcast.
Squads & Team News
|Goalkeepers||Uzoho, Ezenwa, Akpeyi|
|Defenders||Shehu, Ebuehi, Aina, Echiejile, Idowu, Awaziem, Ekong, Balogun, Omeruo, Eze|
|Midfielders||Onazi, Ndidi, Ogu, Agbo, Joel Obi, Etebo, Agu|
|Forwards||Musa, Iheanacho, Simon, Moses, Ighalo, Iwobi, Okechukwu, Ajayi|
Junior Ajayi could be handed his Super Eagles debut against Serbia as Oghenekaro Etebo may be ruled out through injury.
Francis Uzoho is expected to remain in goal ahead of Ikechukwu Ezenwa and Daniel Akpeyi, with William Troost-Ekong forming a solid partnership with Leon Balogun in defence.
Potential Nigeria XI: Uzoho, Shehu, Ekong, Balogun, Idowu, Obi, Ndidi, Iwobi, Iheanacho, Moses, Ighalo
|Goalkeepers||Dmitrovic, Stojkovic, Rajkovic|
|Defenders||Rajkovic, Tosic, Kolarov, Maksimovic, Obradovic, Veljkovic, Rukavina, Nastasic, Ivanovic|
|Midfielders||Matic, Ljajic, Tadic, Kostic, Zivkovic, Maksimovic, Gacinovic|
|Forwards||Mitrovic, Ljajic, Prijovic|
Coach Mladen Krstajic is expected to make a number of changes to the team that started against Morocco.
Nikola Maksimovic is likely to be replaced by Branislav Ivanovic, while Marko Grujic is pushing for inclusion ahead of Nemanja Matic.
Possible Serbia XI: Dmitrovic, Tosic, Basta, Kolarov, Ivanovic, Matic, Kostic, Tadic, Zivkovic, Ljajic, Mitrovic
Betting & Match Odds
Serbia are slight favourites to win the game, with dabblebet placing them at 6/4.
Nigeria are 7/4 to claim victory, with the draw at 21/10.
Nigeria will be hoping to preserve their unbeaten run when they meet Serbia for the first time in London.
The Super Eagles are yet to lose any of their last six international games and will be looking to build on their 1-0 victory over Poland on Friday.
Despite the win, however, Nigeria boss Gernot Rohr wants to see further improvements against a Serbia team ranked 34th in the world.
“This team is a young team but also a team of leaders. If we stay humble and continue to work hard to improve our play we have huge potentials, but beating two Fifa top 10 teams in friendlies doesn’t mean we are now World Cup favourites,” he said in a press conference.
Tuesday's meeting will be Serbia's final outing before their World Cup opener against Costa Rica in June and manager Mladen Krstajic will be determined to sign off with a win.
The Group E outfit were beaten 2-1 by Morocco on Friday, with goals from Hakim Ziyech and Khalid Boutaib sealing defeat for Serbia.