The Barcelona superstar stepped up after Sergio Aguero was fouled in the penalty area, with the scores locked level at 1-1.
However, Messi's penalty was poor, with Hannes por Halldorsson able to palm his effort away.
And, naturally, that led to something resembling a social media meltdown, with many quick to point out that Messi's eternal rival, Cristiano Ronaldo, scored from 12 yards in Portugal's pulsating 3-3 draw with Spain on Friday.
Messi-Ronaldo debate will go on eternally, but I think in last 24 hours, God has weighed in which side of the argument she favors pic.twitter.com/ui1cXx2rp1— roger bennett (@rogbennett) June 16, 2018
Ronaldo beats the best goalkeeper in the world with a free kick and Messi can’t beat a film director with a penalty 😂😂😂😂— Josh 🏴 (@Joshtrich1) June 16, 2018
Lionel Missi! 😯 #ARG— Robin Bairner (@RBairner) June 16, 2018
Messi and his Argentina team-mates could not breach the Icelandic defensive wall for the remainder of the game, either, and were forced to settle for a point from their opening game of the tournament.