However, Pablo Machin's team make the trip to the Stadio Olimpico in poor form, having lost five of their last eight games in all competitions.
Lazio, for their part, have enjoyed back-to-back Serie A wins along with a morale-boosting Coppa Italia success over Inter in recent games and Simone Inzaghi's side are favourites against their La Liga opponents.
Lazio have won nine matches at home in all competitions this season and William Hill price another triumph for the side from the Italian capital at 27/20 (2.35). Sevilla are offered at 21/10 (3.10) with the draw available at 9/4 (3.25).
Lazio will be without Serbian midfielder Sergej Milinkovic-Savic who is out with a groin injury, while top scorer Ciro Immobile is also a major doubt with a thigh strain.
Sevilla are missing winger Nolito, wing-back Aleix Vidal and centre-back Joris Gnagnon.
Sevilla may currently be occupying a Champions League spot in La Liga but their away record is extremely poor.
In the Primera Division they have not won on the road since September and in the Europa League, they lost two of their three away games in the group stages - at Standard Liege and Krasnodar, respectively.
This will be Sevilla's 43rd game of a season that started on July 26, with the Machin's men playing in three qualifying rounds to just reach the group stage of this competition.
Tips and predictions
Immobile will be a big miss for Lazio against his former club, with the Italy international having scored 33 per cent of their goals in Serie A this term.
With the hosts missing their biggest goal threat and Sevilla looking tired, opposing a high scoring game makes sense. Under 2.5 goals is priced at 4/6 (1.66) while both teams score 'no' is offered at 17/20 (1.85).
Lazio have also scored 63% of their Serie A goals in the second half, while Sevilla have netted 66% of their league goals after the break, so backing a goalless first half at 13/10 (2.30) should also be of interest.