Roma's away record in the Champions League is extremely poor, with the Giallorossi's victory at Qarabag last year their only one in their last 14 matches on the road.
Of the eight teams left in the tournament, the Italians are the highest priced to record a win this week at 14/1 (15.00) with bet365.
Lionel Messi began the weekend La Liga game at Sevilla on the bench, but he should be fit enough for a place in the starting eleven.
Sergio Busquets has been missing since picking up an injury against Chelsea in the previous round but could feature on Wednesday.
For Roma, Belgian international Radja Nainggolan is likely to miss out with an injury picked up during the weekend draw with Bologna.
Messi to lead Blaugrana to victory
Barcelona's number ten showed his importance on Saturday night as he came off the bench at 2-0 down and inspired them to a 2-2 draw.
With Messi in the side, the Catalans will feel confident of defeating any team in the world and Roma's away record means they are unlikely to be able to compete at the fortress that is the Camp Nou.
Tips and predictions
Barcelona have scored over 2.5 goals in ten of their last 13 Champions League games at home and a repeat is priced at 5/6 (1.83).
Roma's last visit to the Camp Nou saw them concede six times back in 2015 and the Italians have not kept a clean sheet on the road in this competition since 2007.
Prices correct at time of writing. Please gamble responsibly.