The Blancos have named a 19-man party for the midweek clash at Wembley Stadium.
Bale had been hoping to make the cut, allowing him to face his former club, but he has only been able to return to light training ahead of the contest.
Zinedine Zidane has been forced to overlook him as a result, but Isco does make the grade after shaking off a knock which kept him out of training on Monday.
Isco was seen limping down the tunnel following Real’s shock 2-1 defeat to Girona, but assured reporters after that contest that he would be ready for European action.
Zidane clearly believes that the Spain international is back to full health, but he has a number of other injury issues to contend with.
Keylor Navas, Raphael Varane, Dani Carvajal and Mateo Kovacic are all missing for Real as they prepare to take on Spurs.
The defending champions have also seen club talisman Cristiano Ronaldo hit an uncharacteristic slump which has seen the usually prolific Portuguese struggle for goals.
Zidane will be eager to see a man recently honoured with the FIFA Best Award get back on track in England, while offering him and his team a welcome boost amid a testing run and regular rounds of criticism.
Having swept to a La Liga and Champions League double last season, Real currently sit eight points adrift of arch-rivals Barcelona in the Spanish top-flight and were held 1-1 at home by Tottenham in their last continental outing.