One of Brazil's football giants will line up next year in Serie B for the first time in their history, as Internacional went down on the final day of the season.
The Porto Alegre side needed a victory in their last game against Fluminense and for results elsewhere to go their way in order to beat the drop.
But they were forced into a corner in Rio de Janeiro after an early penalty save from Danilo when Douglas fired the hosts into the lead.
Gustavo Ferrareis gave the Colorado a glimmer of hope with a late equaliser, but it was too little, too late as the club's fate was sealed.
Inter were one of five Brazilian clubs never to have tasted relegation since the start of the national championship in 1971.
Cruzeiro, Flamengo, Santos and Sao Paulo make up the rest of that select group, which will become a quartet when the new season kicks off in 2017.