Coach Dunga confirmed the 23-year-old had been omitted from his group due to injury, following discussions with the Premier League club’s medical staff.
"He had an injury in his last training session with Chelsea," Dunga confirmed.
"We talked to Mourinho and with the Chelsea doctors. His period of recovery will be somewhat prolonged, so he will not be in the best condition. We think he will be better prepared for the World Cup qualifiers, which begin soon after the Copa America."
His absence provides the opportunity for Everton Riberio of UAE-based club Al-Ahli to return to the squad.
Real Madrid’s Marcelo, Monaco’s Fabinho, Casemiro of Porto and Santos striker Robinho were among those breathing a sigh of relief as their places in the squad were all confirmed after what had been a nervous wait.
The rest of the group is made up of those who have featured regularly since the World Cup, including David Luiz, Thiago Silva, and Barcelona star and captain Neymar.
The squad will meet at the Granja Comary training complex in Teresópolis on June 1 ahead of their final warm-up matches against Mexico and Honduras in what will be Brazil’s first games in front of a home crowd since last year’s World Cup.
Dunga was reappointed as Brazil coach after Luiz Felipe Scolari’s World Cup campaign acrimoniously in a 7-1 semi-final defeat to Germany.
The 51-year-old was previously in charge of his country between 2006 and 2010, leading them to the 2007 Copa America title in Venezuela.
The former World Cup-winning captain has led Brazil to eight consecutive Chevrolet Brasil Global Tour victories since his July 2014 appointment.
The Selecao will be looking for their ninth South American crown in Chile, where they have been drawn in Group C alongside Colombia, Peru and Venezuela.
They kick off their campaign on June 14 against Peru at the Estadio Municipal German Becker in Temuco.
Brazil squad in full
Goalkeepers : Jefferson (Botafogo), Diego Alves (Valencia), Marcelo Grohe (Gremio).
Defenders : David Luiz (PSG), Marquinhos (PSG), Thiago Silva (PSG), Miranda (Atletico Madrid), Danilo (Porto), Fabinho (Monaco), Marcelo (Real Madrid), Filipe Luis (Chelsea).
Midfielders : Luiz Gustavo (Wolfsburg), Fernandinho (Manchester City), Elias (Corinthians), Casemiro (Porto), Everton Ribeiro (Al-Alhi), Douglas Costa (Shakhtar Donetsk), Willian (Chelse), Coutinho (Liverpool).
Forwards : Neymar (Barcelona), Diego Tardelli (Shandong Luneng), Robinho (Santos), Roberto Firmino (Hoffenheim).