The green beam was shined towards a number of players, including Lionel Messi – as well as towards Argentina coach Diego Maradona.
The incident has highlighted concerns over security at the stadium, with questions being asked as to how an item such as this was missed when the fans entered through security checkpoints.
A statement issued by FIFA said: “We noticed this incident once and immediately asked the stadium security to look into the matter.
“According to the information that we have at this time, the incident only occurred once. Also at the time of writing, we have not received any comments from any of the teams.”
This comes after a long line of similar laser pen incidents during high-profile matches. Cristiano Ronaldo was also targeted during a Champions League game for Manchester United against Lyon in February 2008.