The Dortmund striker, who only declared for Paraguay last month, scored on his debut last week against Republic Of Ireland before making it two in two against Ivory Coast last night.
Despite his initial heroics, Barrios is expected to start on the bench this summer with Roque Santa Cruz likely to partner Nelson Valdez in attack.
Monterrey midfielder Osvaldo Martinez will probably be the most disappointed of the names left out by Martino but there were no major surprises.
Paraguay have been drawn in Group F alongside New Zealand, Slovakia and Italy.
|Justo Villar||Valladolid (ESP)|
|Aldo Bobadilla||Deportivo Medellin (COL)|
|Diego Barreto||Cerro Porteno|
|Claudio Morel Rodriguez||Boca Juniors (ARG)|
|Denis Caniza||Leon (MEX)|
|Paulo da Silva||Sunderland (ENG)|
|Dario Veron||Pumas (MEX)|
|Julio Cesar Caceres||Atletico Mineiro (BRA)|
|Aureliano Torres||San Lorenzo (ARG)|
|Antolin Alcaraz||Wigan Athletic (ENG)|
|Edgar Barreto||Atalanta (ITA)|
|Cristian Riveros||Sunderland (ENG)|
|Enrique Vera||Liga De Quito (ECU)|
|Jonathan Santana||Wolfsburg (GER)|
|Nestor Ortigoza||Argentinos Juniors (ARG)|
|Roque Santa Cruz||Manchester City (ENG)|
|Nelson Haedo Valdez||Borussia Dortmund (GER)|
|Oscar Cardozo||Benfica (POR)|
|Edgar Benitez||Pachuca (MEX)|
|Lucas Barrios||Borussia Dortmund (GER)|