VVV-Venio midfielder Keisuke Honda and striker Takayuki Morimoto of Italian club Catania are among the 18 named by coach Yasuharu Sorimachi.
Sorimachi wanted 28 year-old international midfielder Yasuhito Endo but the Gamba Osaka star fell sick.
"I chose these 18 players to showcase Japanese football in Beijing. First and foremost, I want to pour all our efforts and focus into going through the group round," he said.
Japan warm-up for Beijing, where they will face Netherlands, Nigeria and USA, with warm-up matches against Australia on July 24 and Argentina five days later.
The tournament last from August 6 -23.
Goalkeepers: Kaito Yamamoto (Shimizu S-Pulse), Shusaku Nishikawa (Oita Trinita)
Defenders: Hiroki Mizumoto (Kyoto Sanga), Yuto Nagatomo (FC Tokyo), Masato Morishige (Oita Trinita), Michihiro Yasuda (Gamba Osaka), Atsuto Uchida (Kashima Antlers), Maya Yoshida (Nagoya Grampus)
Midfielders: Takuya Honda (Shimizu S-Pulse), Hiroyuki Taniguchi (Kawasaki Frontale), Yohei Kajiyama (FC Tokyo), Hajime Hosogai (Urawa Reds), Keisuke Honda (VVV-Venlo/NED), Shinji Kagawa (Cerezo Osaka)
Forwards: Yohei Toyoda (Montedio Yamagata), Tadanari Lee (Kashiwa Reysol), Shinji Okazaki (Shimizu S-Pulse), Takayuki Morimoto (Catania/ITA)