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The Rio and Porto Alegre giants will face off in the biggest clash of the first Copa knock-out phase, while holders Santos meet Bolivar and Velez play against Atletico Nacional

A mouth-watering clash between Brazilian rivals Fluminense and Internacional is the highlight of the draw for the Copa Libertadores last-16, as the group stages ended on Thursday evening.

The seeding system used to determine each qualifiers opponents in the knock-out stages means that the team who finishes the group round with the best record will play the side with the worst record. As a result Fluminense, who won five of their six Group 4 fixtures to finish with 15 points, were drawn against their compatriots from Porto Alegre. Inter managed just two wins from six outings, and only The Strongest's defeat to Santos kept the 2010 winners in the competition after falling to defeat against Juan Aurich.

Elsewhere in the draw, current champions Santos will meet Bolivar while fireworks are expected when Velez Sarsfield take on dangerous Colombian side Atletico Nacional. The Fortin, who went out to Penarol in the 2011 semi-finals, meet Santiago Escobar's free-scoring Medellin outfit and will have to be wary against an attack that netted 16 times during the group phase.

The last-16 will be made up of teams from a total of eight nations, with Brazil providing five of the remaining sides. Boca, Lanus and Velez make up the Argentine contingent, while Ecuador can boast two sides thanks to Deportivo Quito and Emelec's surprise qualification.

Defensor Sporting's elimination on Tuesday means that Uruguay do not have a single participant left in the competition. Bolivar, on the other hand, become the first Bolivian team to make the knock-out stages for 12 years. The La Paz were also the last team to qualify, having reached the quarter-finals of the 2000 edition before going out to America.

LAST-16

Group Winner
Group Runner-Up
 
 Fluminense  Internacional
Corinthians Emelec
Santos  Bolivar
Universidad de Chile Deportivo Quito
 Libertad Cruz Azul 
 Velez Sarsfield   Atletico Nacional
 Lanus  Vasco da Gama
 Union Espanola  Boca Juniors

The first legs of the last-16 are scheduled to be played between May 1-3, with the return fixtures taking place the following week.

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