The Italian champions need just a draw at home to Valencia to ensure their place in the last 16, although whatever happens at the Allianz Stadium, they will be through if Manchester United lose to Young Boys.
Valencia's future in the competition looks much more uncertain with the Spanish side realistically needing wins in their last two matches to keep alive their hopes.
Cristiano Ronaldo was sent off in the first game between these sides in September, but the top scorer in the history of the Champions League looks set to start on Tuesday night.
Federico Bernardeschi msissed the weekend win over SPAL with an abductor problem and remains a doubt. Emre Can has returned to training after thyroid surgery although he too missed Saturday's game and is unlikely to feature.
Valencia have right-back Cristiano Piccini missing with wideman Dennis Cheryshev also out.
Juventus have been the best side in Group G by some distance, even if the current standings do not accurately reflect their superiority.
With ten men they managed to comfortably beat Valencia 2-0 on Matchday One and while Los Che have improved a little since then, there remains a gulf in class between these two sides.
Marcelino Garcia's men have won three matches in a row for the first time this season and have improved their efficiency in front of goal which has been their main problem this term.
They know they have to win in Turin and we could realistically expect them to get a goal, although a victory is likely to be beyond them.
Tips and predictions
There will be plenty of urgency from Valencia who have enough attacking quality to find the back of the net, yet the Allianz Stadium is a real fortress and Juve should generally backed to win when playing at home.
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