Daniel Cardoso opened the scoring with a penalty in the 15th minute, but Walter Musona's strike before the interval ensured a point for Polokwane City.
The home side made a single change from the team that held Mamelodi Sundowns to a 1-1 draw in midweek with Ayanda Nkili the only missing player through suspension.
Amakhosi, on the other hand, changed things around with Kgotso Moleko coming in at the right-back position and Leonardo Castro watching his teammates from distance.
Chiefs were looking to avenge the first round result which saw them lose to Rise and Shine, while they also had one eye on climbing the PSL log in pursuit of the league title.
Cardoso gave Amakhosi the perfect start to the encounter when he converted from the penalty spot inside the opening 15 minutes, much to the delight of the visiting fans out in the stands.
This was Cardoso's third goal from the same position in as many matches as he continues to raise his hand as Amakhosi's first-choice penalty taker.
However, after a long drawn midfield battle, City hit back through Musona, who fired past Daniel Akpeyi from distance.
Akpeyi will no doubt have to shoulder the blame for the goal as he had a clear vision of the ball even before it was struck, only to allow it to slip through and into the back of the net.
A frustrated Ernst Middendorp looked on as Polokwane City fans and players celebrated, and perhaps, he would feel betrayed by the man he brought in to be his No.1 after some costly errors by Virgil Vries in the past.
Hendrick Ekstein squandered a glorious chance to restore Amakhosi's lead midway through the first half, but his attempt was blocked by Motloung inside the box.
The game was very open in the latter stages of the first period as Khama Billiat almost made it 2-1 to Amakhosi, only for his volley to go wide of the target.
Chiefs made their first change of the game at the start of the second half when Middendorp brought in Dumisani Zuma for Ekstein 10 minutes into the half.
Ramahlwe Mphahlele was also introduced in Philani Zulu's place, but even then, Chiefs were unable to break down the Polokwane City defence.
Polokwane City controlled the midfield, but they looked very shaky in defence and Teenage Hadebe nearly fired Amakhosi ahead on the hour-mark as he tested George Chigova with a lobbed attempt.
The Zimbabwean goalkeeper did well to tip the shot over the crossbar to ensure that the scoreline remained 1-1.
Both teams failed to create clear-cut goalscoring chances towards the end of the game, and Middendorp threw on Ryan Moon for Ntiya-Ntiya as he was looking to find the winner.
Zuma nearly stole it for Chiefs six minutes before time, but his glancing header from inside the box was easily saved by Chigova as both sides settled for a share of the spoils.