Asante Kotoko successfully began their title defence on Sunday after eliminating lower-tier side Storm Academy in the round of 64 on Sunday.
The Kumasi-based club could not find the back of the net in regulation time but progressed with 6-5 scoreline through penalties, while Ghana Premier League champions Aduana Stars won 1-0 over Young Apostles thanks to Hainikoye Soumana's strike.
At the Len Clay Sports Stadium, Shafui Mumuni got the only goal for Ashanti Gold who pipped Bechem 1-0, while Techiman Eleven Wonders defeated rivals Techiman City 3-1 and Wa Suntaa FC recorded the heaviest victory after thrashing Sankara FC 8-0 in Tamale.
Hearts of Oak snatched a 1-0 victory over Young Wise after Joseph Esso found a breach and scored the only goal, but Ebusua Dwarfs' terrible campaign in all competitions continued as second-tier side Star Madrid shocked them 1-0 at home.
Scores at a glance
Ashanti Gold SC 1-0 Bechem United
Jeffisi United 0-2 Wa All Stars FC
Aduana stars 1-0 Young Apostle
Storm Academy 0-0 Asante Kotoko
(Asante Kotoko wins 6-5 on penalties) .
Techiman XI Wonders 3-1 Techiman City
Young wise 0-1 Hearts of Oak
Inter Allies 1-0 Immigration FC
Jeffisi United 0-2 Wa All Stars
Oil City 1-2 Medeama
Likpe Heroes 0-0 (3-5 pen) WAFA
Samatex 0-0 (2-4 pen) Karela United
Sankara 0-8 Wa Suntaa FC
Mighty Jet 0-1 Tema Youth
Soccer Intellectuals 0-4 Elmina Sharks
River Plate Atletic Club 0-1 Asokwa Deportivo
New Edubiase 1-0 Thunderbolt FC
Emmanuel FC 0-1 Nania FC
Bebeto 0-0 (3-4 pen) Heart of Lions
Vision FC 0-1 Liberty Professionals
Bolga Soccer Masters 3-0 Zuarungu FC
Biz Paradise 1-1 (5-4 pen) Nzema Kotoko
Dreams FC 4-1 Mepom Vatens Sports Club
Ebusua Dwarfs 0-1 Star Madrid
DC United 0-0 (5-4) BA United
Bibiani Gold Stars 5-2 Enchi All Stars
Unistar Academy 2-0 Suampoman FC
Winneba United FC 2-0 Venomous Vipers
Kintampo Young Talents 4-1 Unity FC
Kenyasi Young Dreams 1-1 (Rained off) Bamboi City FC
Akatsi All Stars 1-0 Agbozume Weavers
Tamale City vs Real Tamale United (postponed)