The list will be whittled down to 21 before the team departs for Gabon for a nine-day pre-tournament camping exercise on Friday.
The Black Starlets drew 2-2 with Tanzania in a preparatory game last month, before twice beating Niger 2-0 in a double-header.
Ghana, who accounted for Burkina Faso and Cote d'Ivoire in the qualifiers, are set to return to the Afcon after a four-year absence.
The team will play in Group A, alongside Gabon, Guinea and Cameroon in the tournament which runs between May 21 and June 4.
Goalkeepers: Michael Acquaye (Wafa), Danlad Ibrahim (Asante Kotoko), Kwame Azziz (Mandela Soccer Academy)
Defenders: Bismark Terry Owusu (Mandela Soccer Academy), Kingsley Owusu (Dreams FC), Najeeb Yakubu (New Town Youth), Nana Kwame Antwi (Accra Young Wise), Rashid Alhassan (Aduana Stars), Razak Yusuf (FC Tanga), Faisal Osman (Kumasi Young Vipers),Gideon Acquah (Accra United), John Otu (Dreams FC)
Midfielders: Isaac Antah (Accra Young Wise), Idriss Mohammed (Cheetah FC), Simon Asamoah Appiah (Wa All Stars), Gabriel Leveh (Tema Youth), Sulley Ibrahim (New Life FC), Emmanuel Toku (Cheetah FC), Edmund Arko Mensah (Wa All Stars), Samuel Monnie Andoh (Zein United)
Strikers: Eric Ayiah (Charity Stars), Mohammed Aminu (WAFA), Kwadwo Opoku (Attram D'Visser), Nathaniel Onyina (Sporting Club), Patmos Arhin (Mandela Soccer Academy)