Atletico Madrid winger Gelson Martins is expected to miss El Derbi at the Santiago Bernabeu on Saturday due to injury after he trained separately from his team-mates during Friday's session due to a bruised knee.
Atleti's Jose Gimenez is also still an injury concern after being withdrawn from Tuesday's clash with Huesca, though Diego Simeone was optimistic about the defender's availability.
The 30-year-old is not expected to play until after the international break , ruling him out for the clash against their cross-town rivals.
Victory for either side could be enough to see them top the table depending on Barca's result when they face Athletic Bilbao on Saturday afternoon.
Real suffered the first defeat of Julen Lopetegui's tenure against Sevilla, 24 hours after Atletico had won their third game in a row against Huesca.
But the ex-Spain boss does not feel the extra preparation time gives Diego Simeone's men a significant advantage ahead of the derby.
"One day less doesn't worry me, I don't think about that," Lopetegui told the media. "We've got good energy and we're ready so that's more important. The motivation is there, you need motivation for games like this.
"We're on good form even though we didn't have a good first half the other day [against Sevilla]. In general, we're in good shape.
"We've been playing every three days recently and I think the second half against Sevilla was good. The side physically is good, other things and football matters come into play but physically no problems.
"I expect a balanced, demanding game that will be hard. We will have to compete, play as well, but compete. We are ready for this game against a very good opponent who will make things hard for us. That's what we expect, a game of the highest level, we have no doubts about that.
"We can't look for excuses now, the calendar is like that. Energy and motivation have to be above all these things and we have that at the moment going into these games."