Ashraf was excluded from Tuesday’s 2-0 victory over Al Ittihad in a Egyptian Premier League match due to exhaustion, but was included in the squad that was due to leave for South Africa on Wednesday.
Also included in the trip was new signing Mahmoud Wahid, who was a doubt for the Sundowns match with a muscle strain and also sat out Tuesday’s match.
Midfielder Hamdi Fathi is also back in the squad that was published on the Al Ahly website after recovering from injury.
Nigerian forward Junior Ajayi, Mohamed Hany and Saad Samir are also in the travelling squad, having been rested from Tuesday’s league game.
But some key injured players remained in Cairo, including goalkeeper Sherif Ekramy, veteran midfielder Walid Soliman, striker Amr Gamal, Ahmed Hamoudi and defender Ahmed Alaa.
Meanwhile, the return leg between Al Ahly and Mamelodi and Sundowns will be played at the Suez Stadium instead of Borg El Arab.
The quarter-final second leg would be played seven days after Saturday’s fixture at the Suez Stadium, which is one of the 2019 Africa Cup of Nations venues.