South Africa head coach Molefi Ntseki has announced the squad which will face Sao Tome and Principe.
The two teams are set to meet in the 2021 Africa Cup of Nations (Afcon) qualification Group C matches later this month.
Kaizer Chiefs captain Itumeleng Khune and Black Leopards full-back Thabo Matlaba have been included in the 25-man squad.
Khune's inclusion has come as a surprise as the 32-year-old has been serving as Chiefs' second-choice goalkeeper behind David Akpeyi.
Boasting 91 Bafana caps, the accomplished player seems to have been included in the squad due to his impeccable experience.
Khune, who hasn't been with Bafana since 2018, has been selected alongside SuperSport United goalkeeper Ronwen Williams and Bidvest Wits shot-stopper Brandon Petersen.
Meanwhile, Matlaba is another experienced player, who is making his return to the national team having played in his last game for South Africa in 2016.
The former Orlando Pirates captain has been a consistent performer for Leopards this season.
Ntseki has also included in-form Chiefs full-back Reeve Frosler, Maritzburg United striker Thabiso Kutumela and Lamontville Golden Arrows defender Gladwin Shitolo in the squad.
While Mamelodi Sundowns midfielder Andile Jali and Chiefs midfield maestro George Maluleka have also been recalled to the national team.
Bafana are expected to host the Falcons and True Parrots Team on the weekend of 27-29 March at FNB Stadium and the return leg will be played four days later in Sao Tome and Principe.
Goakeepers: Itumeleng Khune (Kaizer Chiefs), Ronwen Williams (SuperSport United), Brandon Petersen (Bidvest Wits)
Defenders: Erick Mathoho, Reeve Frosler (Kaizer Chiefs), Buhle Mkhwanazi, Thulani Hlatshwayo, Sifiso Hlanti (Bidvest Wits), Gladwyn Shitolo (Golden Arrows), Innocent Maela (Orlando Pirates), Mosa Lebusa (Sundowns), Thabo Matlaba (Black Leopards)
Midfielders: Andile Jali, Themba Zwane (Mamelodi Sundowns), George Maluleka, Lebogang Manyama (Kaizer Chiefs), Dean Furman, Teboho Mokoena (SuperSport United), Lebogang Phiri (Guigamp), Thembinkosi Lorch (Pirates), Percy Tau (Club Brugge), Thulani Serero (Al Jazira)
Strikers: Lebo Mothiba (Strasbourg), Bradley Grobler (SuperSport United), Thabiso Kutumela (Maritzburg United)