The Neuva Chicago defender was hit in the abdomen while reportedly defending his wife and son outside of his home in Monte Grande, Buenos Aires, and passed away after undergoing surgery at Hospital Santa Maria.
Daniel Ferreiro, vice-present of Neuva Chicago, confirmed the news on Twitter in the early hours of Friday morning, writing: "Regret from the soul to inform that our Rodrigo Espindola died moments ago."
Lamento desde el alma informar que nuestro Rodrigo Espindola falleció hace instantes. Nueva Chicago a traves de su CD se pone a disposción..— Daniel Ferreiro (@dhferreiro22) May 13, 2016
Espindola began his career in the lower Argentine divisions with Chacarita Juniors before later joining Racing Club and then Nueva.
Espindola leaves behind a wife and six-month-old baby.