The veteran goalkeeper has started just two Serie A matches since October due to a calf problem but Wojciech Szczesny has been a capable back-up, with 10 clean sheets in 15 appearances in all competitions.
Head coach Massimiliano Allegri was confident this week he would have Buffon available and he has been included in the 20-man squad for the match, which takes place the day before the goalkeeping great's 40th birthday.
Buffon takes the place of Leonardo Loria but the squad is otherwise unchanged from last Sunday's 1-0 win over Genoa in Turin.
Juventus have won each of their last five away games against Chievo in Serie A, scoring at least two goals in four of these, with Saturday's hosts having scored only two goals in their last seven league games against the Turin side.
Juventus go into the weekend in second place, a point behind Serie A leaders Napoli.