Leonardo Spinazzola is the only first-team member excluded from the 23-man selection as he continues to recover from a long-term knee injury.
Five-time winner Ronaldo brings significant Champions League know-how to a club seeking its first European trophy since 1996.
The 33-year-old is the competition's all-time leading scorer, although he is yet to open his Juve account.
Juventus squad in full:
Goalkeepers: Mattia Perin, Carlo Pinsoglio, Wojciech Szczesny
Defenders: Andrea Barzagli, Mehdi Benatia, Leonardo Bonucci, Joao Cancelo, Giorgio Chiellini, Mattia De Sciglio, Daniele Rugani, Alex Sandro
Midfielders: Rodrigo Bentancur, Federico Bernardeschi, Emre Can, Douglas Costa, Juan Cuadrado, Sami Khedira, Blaise Matuidi, Miralem Pjanic
Forwards: Paulo Dybala, Moise Kean, Mario Mandzukic, Cristiano Ronaldo