With only 23 men in practice the day before, the increase in number was a result of the arrival of Belgium-based Daniel Opare, whose lateness was due to a tardy invite as a replacement for the injured Andy Yiadom, who has withdrawn from this month's international duties.
Twenty-one men were present on the first day of training on Tuesday. The number subsequently boosted by the arrivals of Inter Milan's Kwadwo Asamoah and Italian-born Trabzonspor striker Caleb Ekuban, who is on his debut invite to the Black Stars.
Second in Group F, one point behind leaders Kenya, Ghana are hoping to claim three points on Saturday to finish the qualifying campaign as group winners.
Both sides have already secured qualification tickets for the final tournament in Egypt in June/July.
At the Afcon, the Black Stars will be seeking to end a 37-year trophy drought, having last won the title at Libya 1982.