The first game featured Tunisian champions Esperance and Egyptian football powerhouse Al Ahly at the Stade Olympique de Rades in the Tunisian harbour city of Rades on Friday night.
Al Ahly went into this encounter needing a victory in order to move to the top of the Group A standings having drawn 0-0 with Esperance at home two months ago.
The Red Devils edged out the Blood and Gold 1-0 in the North African Derby with Morocco international Walid Azarou scoring the only goal of the match in the first half.
The result saw record-eight time Champions League winners Al Ahly join Esperance on eight, on the league standings.
However, the Red Devils moved to the top of the table on the head-to-head rule as they had defeated their hosts on the night.
The winners of the group will be determined on match day six which is the final round of the group stage in two weeks time.
Mazembe, who have already won Group B, will lock horns with Group A runners-up.
Former Ugandan champions KCCA were seeking revenge as they had succumbed to a 1-0 defeat to Botswana giants Rollers away two months ago.
Patrick Kaddu proved to be the hero for the Garbage Collectors as his second half goal earned the hosts a 1-0 victory on the day.
As a result, KCCA climbed up to third place on the Group A standings with six points from five games, while Rollers slipped down to third place on the table having collected three points.
Both teams were making their debut appearances in the group stages and they will be hoping to end their campaign with victories next week.
KCCA will travel to Egypt where they are scheduled to face Al Ahly, while Rollers are expected to welcome Esperance in Botswana.
Both games will be played on Tuesday, August 28.