Kickoff of the match at the brand-new facility is yet to be announced, with ESPN and Univision Networks broadcasting the game live.
The U.S. will face the Canaleros in the penultimate match of the Hexagonal, before closing out the 10-game final round of CONCACAF qualifying with a trip to Trinidad and Tobago on Oct. 10.
The game marks only the second time the U.S. has played in Orlando and the first time since 1998. It will be only the second World Cup qualifier for the U.S. in Florida since 1980.
After four matches, Bruce Arena's men sit fourth in the Hexagonal standings, which would be good enough for a playoff berth against an Asian side for a place at Russia 2018.