Neymar thought he had won Brazil a penalty in the 2-0 victory over Costa Rica last week, but the decision was overruled with the help of VAR, referee Bjorn Kuipers instead deeming it a dive.
Guardado has subsequently urged the officials to keep an eye on Neymar in Samara on Monday.
"We all know who Neymar is, but it isn't up to me or my team to judge him, but the referees and FIFA," he said.
"Now there's VAR they need to watch his style and know how to manage it.
"We know he likes to exaggerate fouls, throw himself on the ground a lot, but that's his style of play and the person who needs to stop that is the referee, not us."
Guardado feels optimistic of reaching the quarter-finals, despite Mexico having failed to beat Brazil in any of their four previous encounters at the World Cup.
"There's no greater memory than making history with a fifth match. We're different players in terms of the mental aspect, but we know we'll be judged by whether we get through or not," he added.
"What greater motivation could you need when we face off against the five-time champions in the game of our lives? It's the perfect scene.
"It's the biggest game of our lives."