Nemanja Matic is the latest Manchester United player to withdraw from his national team's upcoming games.
Matic, 30, was forced to miss recent United games against Liverpool, Crystal Palace, Southampton and Paris Saint-Germain before returning to Ole Gunnar Solskjaer's starting line-up for the Premier League defeat to Arsenal.
He was taken off with four minutes remaining of the FA Cup quarter-final loss to Wolves at Molinuex last Saturday.
His withdrawal from the squad appears to be a precautionary measure, with coach Mladen Krstajic telling the Serbian FA official website: “Nemanja Matic will not play in Wolfsburg, nor in Lisbon. He played the last match for his club but did not stay on the field until the end.”
There is also a doubt over the fitness of United striker Romelu Lukaku.
The Belgian joined up with his national squad ahead of their games against Russia on Thursday and Cyprus on Sunday despite a foot injury that kept him out of the Wolves game. He is being assessed by Belgium's medics.
Martial has a knee injury with an official France statement reading: “Anthony Martial is suffering from recurring problems in the right knee. He again felt severe pain in his right knee after Saturday's match against Wolverhampton.”
Shaw's issue is unspecified.
United return to Premier League action against Watford at Old Trafford on March 30 following the international break.