Ernesto Valverde's men were held in a goalless game in Greece on Tuesday, but still top their group by three points from Juventus and remain unbeaten since the Spanish Supercopa in August.
The Blaugrana lead La Liga by four points from Valencia, with Real Madrid and Atletico eight off the pace. So far, Barca have won nine of their fixtures and drawn the other - away to Diego Simeone's side last month.
Ousmane Dembele is still sidelined with a thigh injury, but the French forward posted a picture on social media this week which revealed he is back in training with Barcelona.
Andres Iniesta should be involved after returning to training on Thursday, but Andre Gomes, Sergi Roberto and Rafinha are all sidelined.
Arda Turan is doubtful.
Gerard Pique was suspended for the trip to Olympiacos, but the centre-back returns on Saturday and Barcelona do not have any players banned in La Liga.
BARCELONA POTENTIAL STARTING LINE-UP
Barcelona boss Ernesto Valverde is without Sergi Roberto and Andre Gomes, but Andres Iniesta could return to the starting line-up after recovering from injury.
In defence, Gerard Pique is back after missing the game against Olympiacos through suspension, while Nelson Semedo will start at right-back.
Aleix Vidal is in line to feature further forward in a 4-3-3 formation, the former Sevilla man in contention to start on the right against his former club, with Lionel Messi in a more central role and Luis Suarez drifting in from the left.
SEVILLA TEAM NEWS
Sevilla secured a vital 2-1 win at home to Spartak Moscow in the Champions League on Wednesday night to move back into second place in Group E and erase the memory of their shock 5-1 loss in the Russian capital in the previous matchday.
Doubts remain over new coach Eduardo Berizzo. The former Celta Vigo boss has led the Andalusians to six wins in their 10 matches in La Liga, although they have also lost three times so far, including a 4-0 defeat at Valencia in their last away game, and are in fifth place ahead of Saturday's trip to Camp Nou.
Daniel Carrico, Nico Pareja and Joaquin Correa will all miss the game against Barcelona through injury.
BEST OPTA MATCH FACTS
Sevilla have not won any of their last 14 league games against Barca at Camp Nou, losing 12 of those and conceding 38 goals in the process (2.7 per game).
Barca have only conceded three goals in La Liga this season, their best ever defensive tally after the first 10 games of a season.
The last time Barcelona started a La Liga season with nine wins and a draw was in 2013-14 when they finished second behind Atletico Madrid.
Lionel Messi has scored 29 goals in his 30 games in all competitions against Sevilla, his most favoured opponent in terms of goals scored.
TV COVERAGE & KICK-OFF TIME
Barcelona versus Sevilla kicks off at 20:45 local time at Camp Nou on Saturday and will be broadcast live from 19:40 UK time on Sky Sports Football.