Daylon Claasen netted a second-half brace for the Team of Choice while Samir Nurkovic grabbed a consolation goal for Amakhosi.
Both teams made changes to the teams that played their previous matches with Eric Tinkler bringing back Judas Moseamedi while Daniel Akpeyi took over from Itumeleng Khune in the Chiefs goal.
As expected Chiefs were quick out of the blocks as they dominated ball possession and created goalscoring chances.
However, they were often guilty of squandering those chances with Nurkovic, Leonardo Castro and Khama Billiat the culprits.
Goalkeeper Richard Ofori had to come to his team's rescue in the 24th minute as he made a point-blank save to deny Nurkovic who had connected with George Maluleka's cross into the box.
From there on, it was all Chiefs as they continued to string passes together nicely in midfield without hurting Maritzburg United.
Tinkler's men hardly tested Akpeyi in the first-half but the aim was always to try and frustrate Amakhosi and keep the scoreline at 0-0 for as long as possible.
Amakhosi knew Tinkler had a good record against them because he came into this clash with seven wins, six draws and three defeats to his name against Chiefs since 2014.
And they needed to dig deep in the second-half which they failed to achieve as the visitors punished them for wasting too many goalscoring chances.
Lebogang Manyama should have given Amakhosi the lead in the 64th minute after dribbling his way into the small box, only for Ofori to make a fine save.
Maritzburg United hit back almost immediately at the other end as they capitalised on Akpeyi's mistakes - and Claasen was on hand to make it 1-0 to the Team of Choice.
Claasen doubled his side's lead two minutes later after the Chiefs back four was found in sixes and sevens - Thabiso Kutumela spotted the former Bidvest Wits midfielder and he made no mistake tucking the ball into the back of the net with ease from close range.
Chiefs then pulled one back 12 minutes before the end of the match through Nurkovic but the goal proved to be a consolation as Maritzburg United held on to their lead to claim another big scalp.