The Blaugrana top the table with 75 points from their 29 matches so far and are 11 clear of second-placed Atletico with just nine fixtures remaining.
Before their European games, however, the two teams meet in the Primera Division and here, Goal takes a look at everything you need to know ahead of Saturday's game...
Nelson Semedo is available again after recovering from a hamstring problem, although it may be too early for the right-back to feature at this stage.
Sergio Busquets is still sidelined for a couple more weeks, while Sergi Samper is out with a long-term problem.
Lucas Digne is injured as well, but both Marc-Andre ter Stegen and Gerard Pique should feature despite niggling knee problems.
Barcelona potential starting line-up
Messi is available for the Sevilla match, but looks likely to start on the bench after missing both of Argentina's friendlies with hamstring and adductor problems.
That means Paco Alcacer could start alongside Suarez, who is back from suspension, with Messi kept in reserve until later in the game – particularly with a Champions League clash against Roma to come on Wednesday.
Andres Iniesta is likely to be rested, especially as Philippe Coutinho cannot play in the Champions League for Barca this term, having already done so for Liverpool in 2017-18.
With Busquets still sidelined, Paulinho and Ivan Rakitic are likely to feature in central midfield.
Both Ter Stegen and Pique should start after shaking off their respective knocks.
Sevilla team news
Sevilla surprised many by knocking out Manchester United to reach the quarter-finals of the Champions League earlier this month, beating Jose Mourinho's side 2-1 at Old Trafford after a goalless first leg in Spain.
The Andalusians are in sixth place in La Liga, where their form has been more inconsistent, and they lost 2-1 at Leganes prior to the international break, just a few days after their historic win in Manchester.
Coach Vincenzo Montella is without the suspended Pablo Sarabia for Saturday's game, but the Italian has a fully fit squad at his disposal.
TV channel & kick-off time
Sevilla versus Barcelona kicks off at 20:45 local time on Saturday and will be broadcast live in the UK (United Kingdom) on Sky Sports Main Event and Sky Sports Mix from 19:40 GMT.
In the United States, the game can be watched live on television on beIN SPORTS and kicks off at 14:45 ET.
Best Opta match facts
Sevilla have only won one of their last 21 games against Barcelona in La Liga (drawing four and losing 16 of those). That was a 2-1 success in October 2015. They have also kept a clean sheet in only one of those matches.
The Andalusians have won only two of their six home league games under Montella (drawn one, lost three). They had not lost at all in their previous 21 (eight under Eduardo Berizzo, 13 with Jorge Sampaoli).
Barcelona have hit the woodwork 29 times in La Liga this season, more than any other team in Europe's too five leagues.
The Blaugrana have gone 36 consecutive games without losing in La Liga, only two away from Real Sociedad’s record in 1979-80.
Sevilla are the Liga team that Messi has scored the joint-most goals against (23, along with Atletico). However, only eight of those have come at the Sanchez Pizjuan.