The 26-man roster comprises three goalkeepers, seven defenders, 12 midfielders and four strikers.
Former Atletico Madrid trainee Sadick Adams headlines the squad, which also includes captain Amos Frimpong, veteran midfielder Jordan Opoku and new import Mohammed Vie Sylla.
Kotoko have been pitted against Congolese outfit CARA Brazzaville in the preliminary round of the competition.
Both home and away fixtures set for February, Kotoko will first host their Congolese counterparts in Kumasi.
Goalkeepers: Felix Annan, Michael Abu, Ibrahim Danlad
Defenders: Amos Kwasi Frimpong, Edwin Frimpong Manso, Eric Akomanin Donkor, Augustine Sefah, Awudu Nafiu, Adams Wahab, Emmanuel Owusu
Midfielders: Owusu Jackson, Mohammed Vie Sylla, Kwame Boahene, Douglas Owusu Ansah, Baba Mahama, Jordan Opoku, Seth Opare, Kwesi Nti, Michael Yeboah, Collins Ameyaw, Frank Sarfo Gyamfi, Emmanuel Gyamfi
Strikers: Sadick Adams, Obed Owusu Ampong, Abass Mohammed, Yakubu Mohammed.